Bioconversion of airborne formaldehyde by immobilized formaldehyde dehydrogenase from the recombinant methylotrophic yeast Hansenula polymorpha

نویسندگان

  • Sasi Sigawi
  • Olha Demkiv
  • Galina Gayda
  • Yeshayahu Nitzan
  • Mykhaylo Gonchar
  • Marina Nisnevitch
چکیده

Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed bioreactor was designed to enable a bioconversion of airborne FA by FdDH or by recombinant yeast cells immobilized in calcium alginate beads. The results demonstrated that such the bioreactor with immobilized FdDH provides 97-100% elimination of airborne FA and the bioreactor with immobilized cells eliminates 94-100% of

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تاریخ انتشار 2011